|
|
|
| Registros recuperados: 21 | |
|
|
| Alves,L.P.S.; Almeida,A.T.; Cruz,L.M.; Pedrosa,F.O.; de Souza,E.M.; Chubatsu,L.S.; Müller-Santos,M.; Valdameri,G.. |
| The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99) compared to a standard method based on methanolysis and GC. This method... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Flow cytometry; Nile red; Poly-3-hydroxybutyrate; Herbaspirillum seropedicae; Azospirillum brasilense. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100606 |
| |
|
|
| Rodrigues,Luan F. O. S.; Guimarães,Vandeir F.; Silva,Mônica B. da; Pinto Junior,Artur S.; Klein,Jeferson; Costa,Andreia C. P. R. da. |
| Objetivou-se, neste estudo, verificar o efeito do uso combinado de Azospirillum brasilense, ácidos húmicos e diferentes doses de nitrogênio nas características agronômicas do trigo. Os experimentos foram instalados no dia 21 de maio de 2011 e a colheita foi procedida no dia 13 de setembro de 2011. O delineamento experimental adotado foi o de blocos casualizados em esquema fatorial 4 x 4, com quatro repetições. O primeiro fator se refere à combinação da estirpe Ab-V5 de A. brasilense com os ácidos húmicos, sendo utilizados os seguintes tratamentos: controle; Ab-V5; ácido húmico e Ab-V5+ácido húmico e o segundo fator se refere às doses de nitrogênio (0, 30, 60 e 90 kg ha-1 N). A maioria das variáveis em análise apresentou efeito linear positivo dos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum aestivum L.; Azospirillum brasilense; Substâncias húmicas. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662014000100005 |
| |
|
|
| Souza,Thiago Montagner; Prando,André Mateus; Takabayashi-Yamashita,Cassia Reika; Zucareli,Claudemir; Hirooka,Elisa Yoko. |
| ABSTRACT Chemical composition and contamination of wheat kernels (Triticum aestivum L.) directly affect the quality of the flour obtained from them, determining its acceptability and use by industry. Field trials were conducted to evaluate the effect of agricultural practices on wheat kernel quality (chemical composition and contamination). Cultivation was carried out following maize or soybean (crop succession), with application of nitrogen doses in topdressing (0, 30, 60, 90, and 120 kg ha-1), using seeds inoculated or not inoculated with Azospirillum brasilense. Data were subjected to analysis of variance (ANOVA), comparison of means by Tukey's test (<0.05), and regression to nitrogen doses. Crop succession resulted in changes in kernel chemical... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum aestivum (L.); Crop succession; Nitrogen doses; Azospirillum brasilense; Kernel composition. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000400650 |
| |
|
|
| Benintende,S.; Uhrich,W.; Herrera,M.; Gangge,F.; Sterren,M.; Benintende,M.. |
| El objetivo fue comparar la coinoculación con Bradyrhizobium japonicum y Azospirillum brasilense con relación a la inoculación simple con Bradyrhizobium japonicum en la nodulación, crecimiento y acumulación de N en el cultivo de soja y su rendimiento. Se realizaron dos experimentos en lotes en los que nunca se cultivó soja en años con diferentes condiciones hídricas. Los tratamientos fueron: testigo (sin inoculación), coinoculación con Bradyrhizobium japonicum y Azospirillum brasilense e inoculación simple con Bradyrhizobium japonicum. La nodulación en ambos experimentos fue superior en la coinoculación, aunque hubo diferencias significativas en el experimento con déficit hídrico. Los rendimientos, en ambos experimentos, presentaron valores medios... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coinoculación; Soja; Bradyrhizobium japonicum; Azospirillum brasilense. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2010000200002 |
| |
|
| |
|
|
| Aguirre-Medina,Juan F.; Culebro-Cifuentes,Fernando; Cadena-Iñiguez,Jorge; Aguirre-Cadena,Juan F.. |
| La explotación forestal sin regulación en bosques tropicales promueve la invasión de vegetación secundaria que compite con especies de mediano y lento crecimiento, limitando su regeneración natural. Muchos esfuerzos de reforestación fracasan por esta situación poniendo en riesgo el equilibrio del ecosistema por diferencias en densidad y distribución de especies. Con el fin de promover un mayor crecimiento en menor tiempo de la especie maderable primavera (Tabebuia donnell-smithii Rose) en condiciones de vivero, se evaluó el efecto de la inoculación con Rhizophagus intraradices (Schenck et Sm.) Walker et Schuessler, Glomus spp., y Azospirillum brasilense Tarrand, Krieg et Dóbereiner. El vivero se estableció con semillas recolectadas en el Soconusco,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Azospirillum brasilense; Biofertilización; Rhizophagus intraradices; Glomus; Nitrógeno y fósforo. |
| Ano: 2014 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952014000300008 |
| |
|
|
| Araújo,L.M.; Huergo,L.F.; Invitti,A.L.; Gimenes,C.I.; Bonatto,A.C.; Monteiro,R.A.; Souza,E.M.; Pedrosa,F.O.; Chubatsu,L.S.. |
| Azospirillum brasilense is a diazotroph found in association with important agricultural crops. In this organism, the regulation of nitrogen fixation by ammonium ions involves several proteins including the uridylyltransferase/uridylyl-removing enzyme, GlnD, which reversibly uridylylates the two PII proteins, GlnB and GlnZ, in response to the concentration of ammonium ions. In the present study, the uridylylation/deuridylylation cycle of A. brasilense GlnB and GlnZ proteins by GlnD was reconstituted in vitro using the purified proteins. The uridylylation assay was analyzed using non-denaturing polyacrylamide gel electrophoresis and fluorescent protein detection. Our results show that the purified A. brasilense GlnB and GlnZ proteins were uridylylated by... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Azospirillum brasilense; Nitrogen fixation; PII-like protein; GlnD; GlnB; GlnZ. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000400006 |
| |
|
| |
|
|
| Sotomaior,P.; Araújo,L.M.; Nishikawa,C.Y.; Huergo,L.F.; Monteiro,R.A.; Pedrosa,F.O.; Chubatsu,L.S.; Souza,E.M.. |
| Azospirillum brasilense is a diazotroph that associates with important agricultural crops and thus has potential to be a nitrogen biofertilizer. The A. brasilense transcription regulator NifA, which seems to be constitutively expressed, activates the transcription of nitrogen fixation genes. It has been suggested that the nitrogen status-signaling protein GlnB regulates NifA activity by direct interaction with the NifA N-terminal GAF domain, preventing the inhibitory effect of this domain under conditions of nitrogen fixation. In the present study, we show that an N-terminal truncated form of NifA no longer required GlnB for activity and lost regulation by ammonium. On the other hand, in trans co-expression of the N-terminal GAF domain inhibited the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Azospirillum brasilense; NifA protein; GlnB protein; GAF domain; Nitrogen fixation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200005 |
| |
|
|
| Nishikawa,C.Y.; Araújo,L.M.; Kadowaki,M.A.S.; Monteiro,R.A.; Steffens,M.B.R.; Pedrosa,F.O.; Souza,E.M.; Chubatsu,L.S.. |
| Azospirillum brasilense is a nitrogen-fixing bacterium associated with important agricultural crops such as rice, wheat and maize. The expression of genes responsible for nitrogen fixation (nif genes) in this bacterium is dependent on the transcriptional activator NifA. This protein contains three structural domains: the N-terminal domain is responsible for the negative control by fixed nitrogen; the central domain interacts with the RNA polymerase σ54 co-factor and the C-terminal domain is involved in DNA binding. The central and C-terminal domains are linked by the interdomain linker (IDL). A conserved four-cysteine motif encompassing the end of the central domain and the IDL is probably involved in the oxygen-sensitivity of NifA. In the present study,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Biological nitrogen fixation; Azospirillum brasilense; NifA protein. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200004 |
| |
|
|
| Ishida,M.L.; Assumpção,M.C.; Machado,H.B.; Benelli,E.M.; Souza,E.M.; Pedrosa,F.O.. |
| Two Azospirillum brasilense open reading frames (ORFs) exhibited homology with the two-component NtrY/NtrX regulatory system from Azorhizobium caulinodans. These A. brasilense ORFs, located downstream to the nifR3ntrBC operon, were isolated, sequenced and characterized. The present study suggests that ORF1 and ORF2 correspond to the A. brasilense ntrY and ntrX genes, respectively. The amino acid sequences of A. brasilense NtrY and NtrX proteins showed high similarity to sensor/kinase and regulatory proteins, respectively. Analysis of lacZ transcriptional fusions by the ß-galactosidase assay in Escherichia coli ntrC mutants showed that the NtrY/NtrX proteins failed to activate transcription of the nifA promoter of A. brasilense. The ntrYX operon... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Azospirillum brasilense; Two-component regulatory system; Nitrogen regulation; NtrYX genes; NtrBC genes. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000600004 |
| |
|
| |
|
| |
|
| |
|
|
| Sousa, Ricardo Felipe Braga; Guimarães, Vandeir Francisco; Pinto Jr., Artur Soares; Costa, Andréia Cristina Peres Rodrigues; Offemann, Luiz Claudio; Fuchs, Felipe; Coelho, Gustavo Ferreira. |
| This work aimed to evaluate physiological characteristics of corn seeds provenient from cultivation associated with Azospirillum brasilense and Herbaspirillum seropedicae and reinoculated with Azospirillum brasilense. The seeds were coming from cultivation in field conditions, and the treatments were: T1 - control; T2 - 60 kg hectare-1 N, T3 - inoculation (Azospirillum brasilense - AbV5); T4 - inoculation (Azospirillum brasilense) + 60 kg hectare-1 N; T5 - inoculation (Herbaspirillum seropedicae - SMR1) and T6 - inoculation (Herbaspirillum seropedicae) + 60 kg hectare-1 N. The treatments were divided in two separate blocks, being one reinoculated with A. brasilense (AbV5). The experimental design was completely randomized, in factorial scheme (6x2), being... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Azospirillum brasilense; Herbaspirillum seropedicae; Seeds physiology; Reinoculation; Zea mays. |
| Ano: 2015 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/8753 |
| |
|
|
| Spolaor,Leandro Teodoski; Gonçalves,Leandro Simões Azeredo; Santos,Odair José Andrade Pais dos; Oliveira,André Luiz Martinez de; Scapim,Carlos Alberto; Bertagna,Filipe Augusto Bengosi; Kuki,Mauricio Carlos. |
| ABSTRACT The use of plant growth-promoting bacteria is a promising alternative with low environmental impact to increase the efficiency of use of chemical fertilizers, ensuring high yield with better cost-effective ratio. In maize crops, several studies have demonstrated an increased yield when Azospirillum-based inoculants are used. In the case of popcorn, there are no available studies related to use of inoculation and its response on yield parameters. Thus, the aim of this study was to evaluate the field performance of popcorn when inoculated with the commercial product Masterfix L (A. brasilense Ab-V5 and A. brasilense Ab-V6) and the non-commercial inoculant UEL (A. brasilense Ab-V5 + Rhizobium sp. 53GRM1) associated with nitrogen fertilization. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Azospirillum brasilense; Rhizobium sp.; Inoculants; Grain yield. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000100033 |
| |
|
| |
|
| |
|
| |
|
| |
| Registros recuperados: 21 | |
|
|
|
